Oncology Testing

Fluorescence in situ Hybridization Assays

Available Fluorescence in situ Hybridization Assays

Specimen Requirements
5mL (3mL) Whole blood or Bone marrow in a Dark Green Sodium Heparin tube. Keep specimen at room temperature.  Note: If a Bone Core Biopsy (not the liquid aspirate) is being sent for Chromosomes and or FISH study, the preferred media is RPMI, as this is a nutrient, and it keeps the sample moist and prevents desiccation. Refer to the test name below in the Test Directory for more instructions:  
 
AML, AML1/ETO Translocation 8,21 by FISH
[t(8;21)(q22;q22)]		 Order Name: AML8-21T
Test Number: 9116200
Clinical Use:

Frequently observed karyotic abnormality associated with Acute Myeloid Leukemia (AML), especially FAB M2.
 
AML, CBFB/MYH11, Inversion 16 by FISH
[inv(16) or t(16;16)(p13;q22)]		 Order Name: AML INV 16
Test Number: 9116175
Clinical Use:
inv(16), AML-M4 CBFB/MYH11 is associated with acute myeloid leukemia (FAB M4 Eo subtype)
 
AML, 11q23 Gene Rearrangement by FISH
(11Q23)		 Order Name: AML11Q23
Test Number: 9116225
Clinical Use:
11q23 band (11q23+) bearing the MLL gene translocation (MLL+) is a recurrent chromosome change observed in 3% to 7% of acute lymphoblastic leukemias and in 3% to 4% of acute myeloblastic leukemias.
 
AML, t(15-17), PML/RARA by FISH
[ t(15;17)]		 Order Name: AML 15-17
Test Number: 9116025
Clinical Use:
Useful for diagnosing or excluding acute promyelocytic leukemia. the FISH assay detects that the RARA (17q) region is involved in a translocation with a chromosome other than 15. This indicates a variant translocation which is often resulted from t(11;17) or t(5;17).
 
BCR/ABL (Philadelphia Chromosome) Qualitative by FISH
CML/ALL, bcr/abl, [t( 9,22)]		 Order Name: BCRABL FSH
Test Number: 9113275
Clinical Use:
Useful for diagnosing chronic myelogenous leukemia (CML), following course of treatment in CML, and as a prognostic marker in B-cell acute lymphoblastic leukemia (ALL). In addition, it is useful in excluding CML if other myeloproliferative disorders are in the differential diagnosis.
 
B-Cell Chronic Lymphocytic Leukemia by FISH
[11qA/P, +12, 13q-, 17p-, 6q23 (MYB)]		 Order Name: BCELL CLL
Test Number: 9115660
Clinical Use:
Useful for providing prognostic information in known diagnoses of B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and in multiple myeloma cases.
 
Burkitt's Lymphoma/NHL/ALL, IGH/MYC by FISH
[t(8;14)]		 Order Name: BURKI FSH
Test Number: 9117900
Clinical Use:
Useful to diagnose Burkitt's type lymphoma and some cases of acute lymphoblastic leukemia.
 
CHIC2 (PDGFRA 4q12) by FISH
[(4q12)]		 Order Name: CHIC2 FISH
Test Number: 9115527
Clinical Use:
The PDGFRa fusion (4q12 rearrangement; interstitial deletion of CHIC2 region) is observed in diverse eosinophilia-associated hematologic disorders. Useful in predicting beneficial response to the tyrosine kinase inhibitor imatinib mesylate.
 
Follicular Lymphoma, IGH/BCL2, by FISH
[t(14;18)(q32;18q21.3)]		 Order Name: FOLLIC FSH
Test Number: 9116975
Clinical Use:
Useful to diagnose follicular lymphoma (&gt75%) and some diffuse large B-cell lymphomas.
 
Mantle Cell Lymphoma, IGH/CCND1 by FISH
[t(11;14)]		 Order Name: MANTLE FSH
Test Number: 9116800
Clinical Use:
Useful to detect classical translocation in Mantle cell lymphoma.
 
Multiple Myeloma Profile by FISH
[t(11;14)(CCND1;IGH), 13q14.3, 13q34, t(4;14)(FGFR3;IGH), 11q22.3(ATM), 17p13.1(TP53)]		 Order Name: MULT MYEL
Test Number: 9115585
Clinical Use:
Multiple Myeloma (MM) is characterized by the proliferation of malignant monoclonal plasma cells in the bone marrow. In most cases, there is low proliferation index of terminally different malignant plasma cells. As a result, conventional cytogenetic analysis frequently yields normal results. The most frequent abnormalities with prognostic association have been included in this panel [t(11;14)(CCND1;IGH), 13q14.3, 13q34, t(4;14)(FGFR3;IGH), 11q22.3(ATM), 17p13.1(TP53)] . Interphase FISH studies can enhance the detection rate and complement conventional cytogenetic techniques.
 
Myeloid Disorders Profile by FISH
[-5/5q-, -7/7q-, +8, 20q-]		 Order Name: MYELOID P
Test Number: 9115525
Clinical Use:
Useful for diagnosing some types of myelodysplastic syndromes with certain specific cytogenetic abnormalities (MDS) as well as certain subtypes of MDS such as 5q- syndrome. It is also useful as adjunct test in diagnosing chronic myelomonocytic leukemia (CMML) and juvenile myelomonocytic leukemia (JMML) and for following the evolution of chronic myelogenous leukemia (CML) to accelerated phase or blast crisis.

Oncology Testing