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CD20
Annexin I
CD3
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Recently a new antibody by the name of Annexin I has been added to the immunocytochemistry laboratory. This antibody which is a calcium dependent phospholipid binding protein appears to regulate phospholipid vesical aggregation, mediates the inflammatory response, and inhibits the activity of phospholipase A2.
This antibody is useful in the evaluation of low grade B-cell lymphomas as it appears to have a striking sensitivity and specificity for hairy cell leukemia.
- 1: Lancet. 2004 Jun 5;363(9424):1869-70.
- Simple diagnostic assay for hairy cell leukaemia by immunocytochemical detection of annexin A1 (ANXA1).
- Falini B, Tiacci E, Liso A, Basso K, Sabattini E, Pacini R, Foa R, Pulsoni A, Dalla Favera R, Pileri S.
- Institute of Haematology, University of Perugia, Perugia, Italy. faliniem@unipg.it
A marker capable of distinguishing with certainty between hairy cell leukaemia and other B-cell malignant disease would be of great diagnostic value. Through gene expression profiling, annexin A1 (ANXA1) has been identified as a gene that is upregulated in hairy cell leukaemia. We did immunostaining of 500 B-cell tumours with a specific anti-ANXA1 monoclonal antibody and showed that ANXA1 protein expression is specific to hairy cell leukaemia.
Immunocytochemical detection of ANXA1 represents a simple, inexpensive, highly sensitive and specific (100%) assay for diagnosis of hairy cell leukaemia. This assay will be especially useful in distinguishing hairy cell leukaemia from splenic lymphoma with villous lymphocytes and variant hairy cell leukaemia, both of which usually respond poorly to treatments that are effective in hairy cell leukaemia. PMID: 15183626 [PubMed - indexed for MEDLINE]
Annexin I will also stain T cells, so comparison with the pan T cell marker CD3 in addition to comparison to CD20 is important in its evaluation.
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